通過缺失突變ΔfadR ΔaceF和誘導突變adhE adhR atoC，引入aceF+，為atoB添加啟動子Ptet，改變了大腸桿菌的合成途徑，增加了丁酸產量。

參考文獻：Seregina, T.A., Shakulov, R.S., Debabov, V.G. et al. Construction of a butyrate-producing E. coli strain without the use of heterologous genes. Appl Biochem Microbiol 46, 745–754 (2010).