| 產品名稱 | U937-DC-SIGN |
|---|---|
| 商品貨號 | B179868 |
| Organism | Homo sapiens, human |
| Tissue | pleural effusion |
| Cell Type | lymphocyte |
| Product Format | frozen |
| Morphology | lymphocyte-like |
| Culture Properties | suspension |
| Biosafety Level | 2 [Cells contain SV-40 and CMV viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | histiocytic lymphoma |
| Age | 37 years |
| Gender | male |
| Ethnicity | Caucasian |
| Applications | Study Dengue virus infection, as Dengue virus uses DC-SIGN as an attachment factor. Useful for measuring antibody-mediated neutralization and enhancement in flow cytometry-based neutralization assays. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | The U-937-DC-SIGN cell line was developed by transducing U-937 cells with the gene for human DC SIGN protein (Dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin), a known attachment factor for Dengue viruses. The cloning vector used was pcDNA3 which contains SV-40 and CMV promoter regions. |
| Clinical Data | 37 years
Caucasian
male |
| Antigen Expression | dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN) |
| Comments | This cell line was developed by stably transfecting U-937 cells (ATCC® No. CRL-1593.2™) with pcDNA3-DC-SIGN plasmid. |
| Complete Growth Medium | ATCC® Medium 7318: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
|
| Subculturing | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 to 2 x 105 viable cells/mL. Interval: Maintain cell density between 1 X 105 and 2 X 106 viable cells/mL. Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density). |
| Culture Conditions | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile | Amelogenin: X CSF1PO: 12 D13S317: 10,12 D16S539: 12 D5S818: 12 D7S820: 9, 11 THO1: 6, 9.3 TPOX: 8,11 vWA: 14,15 |
| Name of Depositor | A. Desilva |
| Year of Origin | January 2008 |
| References | Kraus A, et al. Comparison of plaque- and flow cytometry-based methods for measuring dengue virus neutralization. J. Clin. Microbiol. 45(11): 3777-3780, 2007. PubMed: 17804661 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
