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U937-DC-SIGN
U937-DC-SIGN
規(guī)格:
價格:詢價
編號:B179868
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
U937-DC-SIGN
商品貨號
B179868
Organism
Homo sapiens, human
Tissue
pleural effusion
培養(yǎng)基
Cell Type
lymphocyte
Product Format
frozen
Morphology
lymphocyte-like
Culture Properties
suspension
Biosafety Level
2  [Cells contain SV-40 and CMV viral DNA sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
histiocytic lymphoma
Age
37 years
Gender
male
Ethnicity
Caucasian
Applications
Study Dengue virus infection, as Dengue virus uses DC-SIGN as an attachment factor. Useful for measuring antibody-mediated neutralization and enhancement in flow cytometry-based neutralization assays.
Storage Conditions
liquid nitrogen vapor phase
Images
Cell Micrograph of U937-DC-SIGN
Derivation
The U-937-DC-SIGN cell line was developed by transducing U-937 cells with the gene for human DC SIGN protein (Dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin), a known attachment factor for Dengue viruses. The cloning vector used was pcDNA3 which contains SV-40 and CMV promoter regions.
Clinical Data
37 years
Caucasian
male
Antigen Expression
dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN)
Comments
This cell line was developed by stably transfecting U-937 cells (ATCC® No. CRL-1593.2™) with pcDNA3-DC-SIGN plasmid.
Complete Growth Medium
ATCC® Medium 7318: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • an additional 2 mM L-glutamine
    • non-essential amino acids to a final concentration of 0.1 mM
      • 2-mercaptoethanol to a final concentration of 0.05 mM
        • fetal bovine serum to a final concentration of 5%

Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 to 2 x 105 viable cells/mL. 


Interval: Maintain cell density between 1 X 105 and 2 X 106 viable cells/mL.
 

Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density).

Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 10,12
D16S539: 12
D5S818: 12
D7S820: 9, 11
THO1: 6, 9.3
TPOX: 8,11
vWA: 14,15
Name of Depositor
A. Desilva
Year of Origin
January 2008
References

Kraus A, et al. Comparison of plaque- and flow cytometry-based methods for measuring dengue virus neutralization. J. Clin. Microbiol. 45(11): 3777-3780, 2007. PubMed: 17804661

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