Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
sediment from drainage ditch, Lake Waccamaw, North Carolina, 1996
Product Format
frozen
Type Strain
yes
Medium
ATCC® Medium 711: PYB
Growth Conditions
Max Temperature: 45.0°C
Min Temperature: 35.0°C
Duration: grown with mixed bacteria
Protocol: ATCCNO: 50579 SPEC: This strain is routinely shipped as a frozen stabilate. Thaw the ampule, aseptically transfer the material to the surface of a plate of ATCC medium 711 agar (15 x 100 mm), and spread it evenly over the plate using a sterile spread bar or cotton swab. Wrap the edge of the plate with time tape or parafilm and incubate upright at 35C. Within 5-7 days the entire surface of the plate will be covered with cysts. To subculture, rub an area of the surface containing cysts with a sterile cotton swab and then vigorously agitate the swab while it is suspended in 1 ml of sterile distilled water. Aseptically transfer the resulting suspension to the surface of a fresh plate and spread evenly. Incubate as above. Subculture every 14-28 days. This strain is shipped with a mixed bacterial flora, the composition of which is unknown.
Subcultivation
Protocol: ATCCNO: 50579 SPEC: This strain is routinely shipped as a frozen stabilate. Thaw the ampule, aseptically transfer the material to the surface of a plate of ATCC medium 711 agar (15 x 100 mm), and spread it evenly over the plate using a sterile spread bar or cotton swab. Wrap the edge of the plate with time tape or parafilm and incubate upright at 35C. Within 5-7 days the entire surface of the plate will be covered with cysts. To subculture, rub an area of the surface containing cysts with a sterile cotton swab and then vigorously agitate the swab while it is suspended in 1 ml of sterile distilled water. Aseptically transfer the resulting suspension to the surface of a fresh plate and spread evenly. Incubate as above. Subculture every 14-28 days. This strain is shipped with a mixed bacterial flora, the composition of which is unknown.
Name of Depositor
TK Sawyer
Year of Origin
1996
References
Sawyer TK, et al. Learamoeba waccamawensis, n.g., n.sp. (Heterolobosea: Vahlkampfiidae), a new temperature-tolerant cyst-forming soil amoeba. J. Eukaryot. Microbiol. 45: 260-264, 1998.
