Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent).
Incubation: 2 to 5 days at 37°C, a 5% CO2 in air atmosphere is recommended.
Dalldorf G. The coxsackie virus group. Ann. N.Y. Acad. Sci. 56: 583-586, 1953. PubMed: 13139263
Robbins FC, et al. Studies on the cultivation of poliomyelitis viruses in tissue culture. V. The direct isolation and serologic identification of virus strains in tissue culture from patients with nonparalytic and paralytic poliomyelitis. Am. J. Hyg. 54: 286-293, 1951. PubMed: 14877809
Nucleotide (GenBank) : AJ295200 Human coxsackievirus A9B genomic RNA for partial polyprotein gene, isolate VR-186, ATCC.
