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MCP-1
MCP-1
規格:
價格:詢價
編號:B182313
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
MCP-1
商品貨號
B182313
Designations
MCP-1
Species
Homo sapiens, human
培養基
Depositors
M Mehrabian
Applications
A full-length cDNA clone derived as a PCR product from monocyte cDNA. The oligonucleotides used were: 5' CTAACCCAGAAACATCCAATTCTC 3' and 5' GGTGTAATAGTTACAAAATATTCA 3'.
Vector
Construct size (kb): 3.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 0.7379999756813049
Tissue: monocyte
Gene product: cytokine A2, small inducible( monocyte chemotactic protein, monocyte chemotactic protein 1, homologous to mouse Sig-je, small inducible cytokine A2, small inducible cytokine subfamily A (Cys-Cys), member 2, small inducible gene JE) [JE*]
Insert Size (kb)
0.738
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/BamHI--2.7, 0.7; HindIII--3.4; BglII--3.4; PstI--3.0, 0.4; EcoRI/HindIII--2.7, 0.7.
The insert contains the following restriction sites (nt from the 5' end): BglII--178, 271; BalI--204; PstI--382.
Detects a human genomic HindIII fragment of 4.6 kb.
A full-length cDNA clone derived as a PCR product from monocyte cDNA. The oligonucleotides used were: 5' CTAACCCAGAAACATCCAATTCTC 3' and 5' GGTGTAATAGTTACAAAATATTCA 3'.
Does not cross-hybridize to mouse DNA at 65C, 0.1 X SSC, 0.1% SDS.
References

Mehrabian M, et al. Localization of monocyte chemotactic protein-1 gene (SCYA2) to human chromosome 17q11.2-q21.1. Genomics 9: 200-203, 1991. PubMed: 2004761

Yoshimura T, et al. Human monocyte chemoattractant protein-1 (MCP-1). Full-length cDNA cloning, expression in mitogen-stimulated blood mononuclear leukocytes, and sequence similarity to mouse competence gene JE. FEBS Lett. 244: 487-493, 1989. PubMed: 2465924

Margarete Mehrabian, personal communication

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