黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > hTERT RPE-1 B183000
hTERT RPE-1
hTERT RPE-1
規格:
價格:詢價
編號:B183000
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
hTERT RPE-1
商品貨號
B183000
Organism
Homo sapiens, human
Tissue
Retina, eye; pigmented epithelium
培養基
Cell Type
Epithelial cells immortalized with hTERT
Product Format
frozen
Morphology
Epithelial-like
Culture Properties
Adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Normal
Gender
Female
Storage Conditions
Liquid nitrogen vapor phase
Karyotype
This is a near-diploid human cell line of female origin with a modal chromosome number of 46 that occurred in 90% of the cells counted. The sex chromosomes consist of a karyotypically normal X-chromosome and a derivative X-chromosome with additional chromosomal material at the terminal end of the q-arm. The derivative X-chromosome was present in all of the cells analyzed.
Images
Derivation

The hTERT-immortalized retinal pigment epithelial cell line, hTERT RPE-1, was derived by transfecting the RPE-340 cell line with the pGRN145 hTERT-expressing plasmid (ATCC MBA-141). 

Cells were cultured in medium containing hygromycin B until stable clones were selected [Pubmed: 9454332, 9916802].

Antigen Expression
The cells express the Ep-16 antigen as determined by flow cytometry using the Ep-16 monoclonal antibody (ATCC HB-155), and cytokeratins as determined by immunocytochemistry using a pan-cytokeratin antibody.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006.To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 0.01 mg/ml hygromycin B

Subculturing
Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Hanks-Balanced Salt Solution (HBSS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 3.0 to 5.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 10.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 4 x 103 to 6 x 103 viable cells/cm2 is recommended.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: 1:5 to 1:10 twice weekly
Medium Renewal: Every 2 days (or as needed)
Note: Subculture when cell concentration reaches between 2 X 104 and 4 X 104 cells/cm2.

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005

Cryopreservation
Complete growth medium supplemented with an additional 60% fetal bovine serum and 10% DMSO. Store in liquid nitrogen vapor. Avoid immersing vials into liquid nitrogen.
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 12,14
D13S317: 11,12
D16S539: 11
D5S818: 11
D7S820: 10,11
THO1: 9
TPOX: 8
vWA: 17,18
Population Doubling Level (PDL)
As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation.
Name of Depositor
Geron Corporation
Deposited As
Homo sapiens
References

Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Jiang XR, et al. Telomerase expression in human somatic cells does not induce changes associated with a transformed phenotype. Nat. Genet. 21: 111-114, 1999. PubMed: 9916802

Matsunaga H, et al. Beta-galactosidase histochemistry and telomere loss in senescent retinal pigment epithelial cells. Invest. Ophthalmol. Vis. Sci. 40: 197-202, 1999. PubMed: 9888444

Freshney RI. Culture of Animal Cells: A Manual of Basic Technique, 5th edition. New York: Wiley Liss; 2005. For more information on enzymatic dissociation and subculturing of cell lines see Chapter 13.

Hay RJ, Caputo JL, Macy, ML, Eds. (1992) ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Erfle H, et al. Reverse transfection on cell arrays for high content screening microscopy. Nat Protoc?2(2):392-9, 2007. PubMed: 17406600

Kabeche L, Compton DA. Cyclin?A?regulates?kinetochore?microtubules?to?promote?faithful?chromosome?segregation. Nature?502(7469):110-3, 2013. PubMed: 24013174

Erfle H, et al. Work?flow?for?multiplexing?siRNA?assays?by?solid-phase?reverse?transfection?in?multiwell?plates. J Biomol Screen?13(7):575-80, 2008. PubMed: 18599879

Fink J, et al. Comparative?study?and?improvement?of?current?cell?micro-patterning?techniques. Lab Chip?7(6):672-80, 2007. PubMed: 17538708

  • 王經理
    手機號：17280875617
    QQ：1438578920
  • 胡經理
    手機號：13345964880
    QQ：2438244627
  • 周經理
    手機號：17757487661
    QQ：1296385441
  • 于經理
    手機號：18067160830
    QQ：2088210172
  • 沈經理
    手機號：19548299266
    QQ：2662369050
  • 李經理
    手機號：13626845108
    QQ：972239479
  • 曹經理
    手機號：18662422060
    QQ：3671869992