The insert contains 2 HindIII sites at positions 402 and 1475 of the published sequence. Restriction digests of the clone give the following sizes (kb): PstI--4.3, 1.8; EcoRI--6.2; HindIII--4.0, 1.1, 1.0; BamHI--6.2. This insert contains the entire 3' terminal region and lacks 16 nucleotides of the 5' terminal region. It encodes an open reading frame for 559 amino acids from nucleotide 22 to 1698. |
