Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Strain E18 transformed with pFATPOT
Production of site specific alpha-1-antitrypsin
The POT1 sequence can be removed from pFATPOT by a SalI/BamHI double digest as a fragment of approximately 1.6 kb.
The wild-type alpha-1-antitrypsin gene can be removed by a BamHI/XbaI double digest.
The alpha-1-antitrypsin gene is under the regulation of the Saccharomyces cerevisiae TPI promoter, and is followed by the Saccharomyces cerevisiae TPI terminator.
The vector contains a pMB1 and 2 micron replicon.
References
Murray MJ, Kelly JD. Expression of biologically active PDGF analogs in eucaryotic cells. US Patent 4,766,073 dated Aug 23 1988
Insley MY, Kawasaki G. Site specific mutagenesis in alpha1-antitrypsin. US Patent 4,711,848 dated Dec 8 1987
Murray MJ, Kelly JD. Biologically active mosaic proteins. US Patent 4,849,407 dated Jul 18 1989
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
