| Comments |
Animals were immunized against 1D2, a BALB/cJ mouse IgG1 monoclonal antibody specific for ricin A chain. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The 44-6-1 (ATCC CRL-2491) and 44-26-2 (ATCC CRL-2492) cell lines produce antibodies that bind specifically to an idiotypic determinant on 1D2 anti-ricin monoclonal antibody. The 44-6-1 and 44-26-2 monoclonals conjugated to keyhole limpet hemocyanin (KLH) have been shown to induce anti-ricin antibodies in mice. |
| References |
Sieckmann DG, et al. Development of an anti-idiotypic vaccine for ricin. J. Allergy Clin. Immunol. 99: S286, 1997.
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
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