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Distribution host: Escherichia coli HB101 (ATCC 33694)

Size (kb): 5.5000000000000000

DESCRIPTION OF VECTOR:
Intact vector size: 5.500
Type of vector: plasmid
Cloning sites: AflII SpeI SacII NruI ClaI NotI NdeI SnaBI HpaI KpnI
Polylinker sites: AflII SpeI SacII NruI ClaI NotI NdeI SnaBI HpaI KpnI
Construction: pBR322, pSL1190, pRcCMV
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: HIV-1 LTR
marker(s): ampR, neoR, G418R
promoter: HIV-1 LTR
replicon: pMB1
terminator: SV40

Vector: pSupexp (plasmid)

Promoters: Promoter HIV-1 LTR

Construction: pBR322, pSL1190, pRcCMV

Marker(s):G418R,ampR,neoR

Construct size (kb): 5.5

Features: marker(s): ampR, neoR, G418R
promoter: HIV-1 LTR
replicon: pMB1
terminator: SV40
enhancer: HIV-1 LTR

expression vector

Restriction digests of the clone give the following sizes (kb): HindIII--5.5; NotI--5.5; KpnI--5.5.

The AflII and SpeI cloning sites are recommended for the 5' end of foreign DNA to prevent potential translation effects of ATG sequences in the multiple cloning site.

Shuttle expression vector permitting levels of protein production in human cells high enough for immunoprecipitation.

Contains two mammalian transcription units: the foreign DNA regulated by HIV-1 LTR and an SV40 polyadenylation sequence, and HIV-1 tat and neo regulated by an SV40 early promoter and polyadenylation sequence.

The order of the major features in this plasmid is: AatII - HIV-1 LTR - MCS - SV40 polyadenylation - SV40 early promoter - tat - neoR - SV40 polyadenylation - pMB1 ori - ampR.

Temperature: 37.0°C