| Vector |
Construct size (kb): 250.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pTYAC1
Intact vector size: 9.000
Type of vector: YAC
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
restriction site: EcoRI
other: telomere, Tetrahymena, <-
marker(s): URA3
centromere: CEN4
replicon: ARS1
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D7S427
Gene name: DNA Segment, single copy
Contains complete coding sequence?: U
Chromosome: 7; Localization: 7 q36.3-qter
Type of DNA: genomic
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 240.0
Cross references: Insert lengths(kb): 240.0 Gene product: DNA Segment, single copy [D7S427] Alleles: A1, A2, A3, M1, M2, T1, T2, M1, H2-1, H2-2, H2-3, H3-1, H3-2, H3-3, H3-4, H3-5, H1-1, H1-2, H1-3, M2, H2-4 Target Gene: DNA Segment, single copy |
| Comments |
Half-YAC containing the human 7q telomere. This insert is also available in two contigs of cosmid clones, sCos146(2), (3), (6), (14), (19), (30), (48), (49) (ATCC 99434, 99436, 99438, 99440, 99442, 99444, 99446, 99448. |
| References |
Dietz-Band J, et al. Characterization of polymorphic loci on a telomeric fragment of DNA from the long arm of human chromosome 7. Genomics 8: 168-170, 1990. PubMed: 2081591
Riethman HC, et al. Cloning human telomeric DNA fragments into Saccharomyces cerevisiae using a yeast-artificial-chromosome vector. Proc. Natl. Acad. Sci. USA 86: 6240-6244, 1989. PubMed: 2668959
Riethman HC, et al. Physical analysis of the terminal 240 kb of DNA from human chromosome 7q. Genomics 17: 25-32, 1993. PubMed: 8406463
Macina RA, Riethman HC. Mapping vector-adjacent DNA from a 7q human telomeric YAC using RARE. Mamm. Genome 5: 596, 1994. PubMed: 8000150
Helms C, et al. Closure of a genetic linkage map of human chromosome 7q with centromere and telomere polymorphisms. Genomics 14: 1041-1054, 1992. PubMed: 1478646
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