| Vector |
Construct size (kb): 4.75 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, <-, 100-556
insert detection: lacZ', <-, 594-1119
primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, <-
promoter: lac, <-, 1164-1192
replicon: pMB1, <-, 1518-1518
marker(s): ampR, <-, 2276-3136
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SNRPD*
Gene name: small nuclear ribonucleoprotein, SM-D (lupus autoantigen)
Contains complete coding sequence?: U
Chromosome: 15; Localization: 15, 10.19 cR
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.5
Cross references: DNA Seq. Acc.: J04615; EST: EST06410
Nucleotides 1-304 of the insert correspond to
nucleotides 1326-1023 of J04615 with 99% identity.
DNA Seq. Acc.: T08518; EST: EST06410; Primer: M13 Reverse;
THC id: THC122881 Insert lengths(kb): 1.5 Tissue: infant brain, 3 months old (from Bento Soares) Gene product: small nuclear ribonucleoprotein, SM-D (lupus autoantigen) [SNRPD*] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--4.6; HindIII/NotI--3.2, 1.08, 0.44. Based on the insert size and the alignment with GenBank accession J04615 (1326 nt, CDS 466-1188), this clone appears to contain most if not all of the coding region and the 3' untranslated region. |
