| Genotype |
MATalpha ade2 arg1 aro7-1(amber mutation) gal4-69 his3 leu2-1(ochre mutation) lys4 met4 pet17 prt1 rad1 trp4 ura2 galx
(Ochre and amber markers are UAA- and UAG-suppressible, respectively. The ade6 mutation is suppressed by certain omnipotent suppressors. Several of the markers present in the YGSC mapping strains are either amber-suppressible (such as trp1, ade5, aro1D, and rad50-1) or ochre-suppressible (such as ilv1, lys1, ura4, etc.), but no effort has been made to identify them as such here. ) |
| Comments |
Mapping strains: multiply-marked strains from B.I. Ono (AB6) These strains were developed primarily for mapping UAA and UAG suppressors, choosing markers that are approximately 30 cM apart. prt strains are temperature-sensitive; they grow at 23C but not at 36C. pet strains are unable to grow on non-fermentable substrates. aro7 strains require phenylalanine plus tyrosine. gal strains may turn yellow. ade2 strains turn red when present as the sole adenine mutation in the strain. rad1 strains are UV-sensitive. galx is linked to ade2. |
| References |
Ochre and amber markers are UAA- and UAG-suppressible, respectively. The ade6 mutation is suppressed by certain omnipotent suppressors. Several of the markers present in the YGSC mapping strains are either amber-suppressible (such as trp1, ade5, aro1D, and rad50-1) or ochre-suppressible (such as ilv1, lys1, ura4, etc.), but no effort has been made to identify them as such here.
B. I Ono, personal communication
Ochre and amber markers are UAA- and UAG-suppressible, respectively. The ade6 mutation is suppressed by certain omnipotent suppressors. Several of the markers present in the YGSC mapping strains are either amber-suppressible (such as trp1, ade5, aro1D, and rad50-1) or ochre-suppressible (such as ilv1, lys1, ura4, etc.), but no effort has been made to identify them as such here.
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