| 產品名稱 |
pSON8-JUN |
| 商品貨號 |
B183708 |
| Designations |
pSON8-JUN |
| GenBank Number |
J04111
|
| Species |
Homo sapiens, human |
| Depositors |
SJ Elledge |
| Applications |
SOS recruitment system (SRS) is a genetic screening method to detect proteins interacting in the cytoplasm. Positive control for SOS Recruitment System. |
| Vector |
Construct size (kb): 9.694000244140625 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSON8
Intact vector size: 9.469
Type of vector: plasmid
Vector end: NcoI
Vector end: SalI
Cloning sites: NcoI SmaI HpaI SalI EagI
Polylinker sites: NcoI SmaI HpaI SalI EagI HindIII MunI EcoRI XhoI
Other unique sites: ApaI SpeI NheI BglII KpnI BamHI PvuI XbaI AhaII
Construction: pUC
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->
promoter: ADH, ->
MCS: NcoI...EagI, ->
coding sequence: SOS (human Son of sevenless), ->
terminator: ADH, ->
replicon: pMB1
marker(s): ampR, <-
replicon: 2 micron ori
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: JUN
Gene name: c-jun oncogene
Contains complete coding sequence?: N
Chromosome: 1; Localization: 1p32-31
Type of DNA: cDNA
Insert 5' end: NcoI
Insert 3' end: SalI
Insert size (kb): 0.257
Cross references: DNA Seq. Acc.: J04111 Insert lengths(kb): 0.2569999992847443 Gene product: c-jun oncogene [JUN] |
| Insert Size (kb) |
0.257 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): SalI--9.7; HindIII--6.0, 3.7; BamHI--9.7; NcoI/SalI--9.5, 0.25. SOS recruitment system (SRS) is a genetic screening method to detect proteins interacting in the cytoplasm. It is based on membrane targeting with a myristoylation signal and SOS-based activation of Ras protein. Positive control for SOS Recruitment System. The insert contains the leucine zipper motif. |
| References |
Aronheim A, et al. Isolation of an AP-1 repressor by a novel method for detecting protein-protein interactions. Mol. Cell. Biol. 17: 3094-3102, 1997. PubMed: 9154808
|
