Addition of cycloheximide recommended for passage in cell culture.
Biosafety Level
3
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
turkey spleen, Texas
Product Format
frozen
Storage Conditions
-70°C or colder
Comments
Centrifugation (2000 X g for 1 hr.) and addition of cycloheximide recommended for passage in cell culture. *Requests for this agent must carry signed statement assuming all risks and responsibility for lab handling.
Triturate fluid several times to disperse chlamydia. For cell culture, centrifuge inoculumn, adsorb for 1 hour and feed with HEPES buffered culture medium.
Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus.
