| 產品名稱 |
p3ACSF-69 |
| 商品貨號 |
B184054 |
| Designations |
p3ACSF-69 |
| Species |
Homo sapiens, human |
| Depositors |
Genetics Institute, Inc., GG Wong, Genetics Institute, Inc. |
| Applications |
in another host, produces protein colony stimulating factor 1 (macrophage) |
| Vector |
Construct size (kb): 9.0 |
| Insert |
DNA: cDNA Insert lengths(kb): 3.799999952316284 Tissue: trophoblast tPA-30-1 cell line Gene product: colony stimulating factor 1 (macrophage) [CSF1] Target Gene: colony stimulating factor 1 (macrophage) |
| Insert Size (kb) |
3.800 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Shipped: ?Freeze dried Escherichia coli containing the plasmid. |
| Comments |
An EcoRI/XhoI double digest excises the insert in 2 fragments of about 2.0 kb and 1.8 kb. There is an EcoRI site in the insert. Restriction digests of the clone give the following sizes (kb): EcoRI--7.6, 2.1; HindIII--8.8, 0.9. The coding region of this sequence differs from pcCSF-17 (ATCC 53149) by the insertion of 894 base pairs between nucleotides 721 and 722 of the latter (preserving the reading frame). The sequences diverge after the termination codon. This sequence contains an open reading frame of 1662 nucleotides encoding 554 amino acids, as well as 2200 nucleotides 3' non-coding sequence. The vector pXM is a mammalian cell expression vector. When this plasmid is introduced into COS-1 cells, it directs high-level expression of biologically active CSF-1. |
| References |
Clark SC, Wong GG. Hematopoietic growth factors. US Patent 4,868,119 dated Sep 19 1989
Wong GG, et al. Human CSF-1: molecular cloning and expression of 4-kb cDNA encoding the human urinary protein. Science 235: 1504-1508, 1987. PubMed: 3493529
Gordon G Wong, personal communication
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
