| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--4.5, 4.5 (doublet); EcoRV--7.1, 1.9; HindIII--6.6, 2.4. The insert contains the following restriction sites (approximate kb from the 5' end): BglI--1.36; BglII--1.78; EcoRV--1.15, 3.00; HindIII--1.90; PstI--1.09, 1.51, 2.75, 2.95; PvuI--2.73, 3.08; PvuII--1.31, 2.35, 2.72; SalI--0.93, 1.40; XhoI--0.46, 1.44. |