| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--8.6, 6.6, 4.8, 3.0, 2.8, 2.7, 2.15, 1.7, 1.5, 1.25, 1.2, 0.8; BamHI--20.0, 13.0, 8.6, 5.0; PstI--8.4, 5.65, 5.05, 3.8, 3.55, 3.4, 2.8, 2.4, 1.8, 1.65, 1.4, 0.85; HindIII--8.0, 6.8, 6.0, 5.7, 3.6, 3.4, 2.0 (doublet), 1.1. Codominant segregation observed in 60 families. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Enzyme(s) not detecting polymorphism: RsaI, MspI. |
