Restriction digests of the clone give the following sizes (kb): EcoRI--4.1, 2.3; HindIII--4.1, 2.3; As resistance levels may vary, it's important to determine the proper antibiotic concentration for each strain or isolate. One of a series (ATCC 37653 through 37657) of vectors for in vitro mutagenesis (via the omega interposon) of gram-negative genera. pHP45:omega was cut with BssHII+SphI (removing the spcR/strR gene) and the ends were made blunt. This was ligated to a blunt-end 2.8 kb HindIII-PstI fragment of pKT210 containing the chloramphenicol resistance gene. |
