| Insert |
DNA: genomic Insert lengths(kb): 1.399999976158142 Gene product: DNA Segment, single copy [D2S3] Alleles: C4, J10, A1, B1, B2, C1, C2, C3, D2, G4, J1, J3, J4, J5, J6, J7, J8, K2, A2, D1, E1, E2, G1, G2, G3, J2, J9, K1 |
| Comments |
Constructed by inserting Sau3A fragment into BamHI site. Insert contains KpnI sites. Restriction digests of the clone give the following sizes (kb): KpnI--6.0, 0.62; EcoRI--5.3, 1.4; HindIII--6.7; SalI--6.7; BamHI--6.7. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The insert was derived from cosmid 1-5. The most efficient detection of RFLPs at the D2S3 locus is by use of the cosmid 1-5 subclones p5-1-25 (ATCC 59684) and p5-2-96 (ATCC 59000). Use of p5-1-30 (ATCC 59006) and p5-1-32 (ATCC 59002) probably adds little to the PIC. |
| References |
Litt M, White RL. A highly polymorphic locus in human DNA revealed by cosmid-derived probes. Proc. Natl. Acad. Sci. USA 82: 6206-6210, 1985. PubMed: 2994065
Demenais F, et al. Robustness and power of the unified model in the analysis of quantitative measurements. Am. J. Hum. Genet. 38: 228-234, 1986. PubMed: 3946424
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