Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: ScaI Vector end: ScaI Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range:Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749
Insert
Genome: bacteriophage P1
Gene symbol: cre Gene name: recombinase, site-specific Contains complete coding sequence?: U Type of DNA: genomic Insert end: PvuII Insert end: PvuII Insert size (kb): 1.6 Cross references:
Gene product: recombinase, site-specific [cre]
Target Gene: recombinase, site-specific
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Related Products
Component of:ATCC 77369
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Freeze-dried E. coli 1046
Comments
This E. coli contains the pCRE1 plasmid and expresses the Cre recombinase from a lacZ promoter. Insert-containing lambdaMGU2 (77367) cannot grow productively in this RecA- strain, so excised phagemid are recovered as ampR surviviors of phage infection.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 2.05; XmnI--3.1, 2.4, 0.45; HindIII--5.2, 0.75; BamHI--4.4, 1.55; EcoRI/PstI--3.5, 1.4, 0.7, 0.35.
References
Maruyama IN, Brenner S. A selective lambda phage cloning vector with automatic excision of the insert in a plasmid. Gene 120: 135-141, 1992. PubMed: 1327972
