Restriction digests of the clone give the following sizes (kb): EcoRI--8.7; PstI--6.4, 1.35, 1.2; SmaI--7.0, 1.8. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Uses interruption of lacZalpha. Shuttle vector permitting visual detection of recombinants. The partition locus from Agrobacterium plasmid pTAR stabilizes the plasmid in X. citri and X. campestris pvs. campestris, vesicatoria, malvacearum, and translucens grown in broth culture. The order of the major features in this plasmid is: lacZalpha with MCS (pUC18) - oriW (pSa747, IncW) - cos (pHC79) - parA+ (pUCD550) - aphA (pUC4Kan) - mob(P) (pLAFR3, Mob+). |
