| Vector |
Construct size (kb): 3.650000095367432 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D0S1075E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: N
Sequence homology: Homo sapiens ATPase, Ca++ transporting
Genome: Homo sapiens
Gene name: ATPase, Ca2+ transporting, plasma membrane, isoform 2
Contains complete coding sequence?: N
Chromosome: 3; Localization: 3, cR
Tissue: hippocampus, female, 2 years, oligo-dT and random-primed
(Stratagene 936205), prescreened
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 0.6
Cross references: DNA Seq. Acc.: M78075; EST: EST01663; Primer: M13 Forward;
THC id: THC78947
DNA Seq. Acc.: M97260
Nucleotides 1-600 of the insert correspond to
nucleotides 2673-3272 of M97260.
DNA Seq. Acc.: T07949; EST: EST05840; Primer: M13 Reverse;
THC id: THC78947 Insert lengths(kb): 0.6000000238418579 Tissue: hippocampus, female, 2 years, oligo-dT and random-primed (Stratagene 936205), prescreened Gene product: ATPase, Ca++ transporting [ATP2A*] |
| References |
Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749
Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434
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