| Insert |
DNA: genomic Insert lengths(kb): 4.5 Gene product: DNA Segment, single copy [D9S14] Alleles: R5, R1, R2, R6, R3, R4 |
| Comments |
Restriction digests of the clone give the following sizes (kb): AccI--4.6, 1.95; BamHI--2.75, 2.25, 1.35; EcoRI--3.5, 3.3; HindIII--5.1, 1.7; PstI--3.2, 1.2, 0.81, 0.56, 0.36. 0.32. Bands detected after a MspI digest are faint. RsaI digest demonstrates the polymorphism best. The probe should be hybridized to total human DNA (50-100 ug/ml) for 2 h before incubation with the filter (42C) because the probe contains repetitive DNA. Enzyme(s) not detecting polymorphism: BglII, PstI, PvuII. |
| References |
Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pMCT96.1 and pMCT96.2) on chromosome 9q [D9S14]. Nucleic Acids Res. 16: 9881, 1988. PubMed: 2903483
Peter J O'Connell, personal communication
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