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L1.84
L1.84
規格:
貨期:
編號:B185234
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 L1.84
商品貨號 B185234
Designations L1.84
Species Homo sapiens, human
Depositors P Devilee
Vector
Construct size (kb): 4.68
Insert
DNA: genomic
Insert lengths(kb): 0.680
Tissue: placenta
Gene product: DNA Segment, repetitive [D18Z1]
Insert Size (kb) 0.680
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.68, 0.68; HindIII--4.3; BamHI--4.3; PstI--3.5, 0.7; PvuII--uncut.
This represents a variant of alphoid DNA repeat with an estimated copy number of approximately 2000 per haploid genome.
With less stringent washing conditions, the probe detects some signal on chromosomes 2, 8, 9, and 20 in addition to the strong signal on 18.
EcoRI multimers of 4, 6, 8, 10, and 12 segregate with chromosome 18 in mapping panels.
References

Devilee P, et al. Two subsets of human alphoid repetitive DNA show distinct preferential localization in the pericentric regions of chromosomes 13, 18, 21. Cytogenet. Cell Genet. 41: 193-201, 1986. PubMed: 3011362

Devilee P, et al. Sequence heterogeneity within the human alphoid repetitive DNA family. Nucleic Acids Res. 14: 2059-2073, 1986. PubMed: 3960717

Choo KH, et al. A survey of the genomic distribution of alpha satellite DNA on all the human chromosomes, and derivation of a new consensus sequence. Nucleic Acids Res. 19: 1179-1182, 1991. PubMed: 2030938

Alexandrov IA, et al. Chromosome-specific alpha satellites: two distince families on human chromosome 18. Genomics 11: 15-23, 1991. PubMed: 1765373

梅經理 17280875617 1438578920
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