Restriction digests of the clone give the following sizes (kb): BamHI--2.8, 2.1; SmaI--4.7; PstI--4.7; EcoRI--4.l, 0.72; ClaI--4.7.
Mammalian shuttle expression vector containing a CAT (chloramphenicol acetyltransferase) gene expressed under an SV40 promoter. To be used as a control for promoter activity in conjunction with the promoter cloning plasmid KS+SV0CAT (ATCC 37608).
Constructed by inserting the AccI-ApaI fragment of pSV2cat into the EcoRV site of Bluescript KS+. Ends were blunt.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Tsonis PA, et al. CAT constructs with convenient sites for cloning and generating deletions. Nucleic Acids Res. 16: 7745, 1988. PubMed: 3166134
