| 產(chǎn)品名稱 |
Influenza B virus |
| 商品貨號(hào) |
B185431 |
| Classification |
Orthomyxoviridae, Influenzavirus B |
| Agent |
Influenza B virus |
| Strain |
B/Virginia/ATCC4/2009 |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen 1 mL per vial |
| Storage Conditions |
-70°C or colder |
| Images |
 |
| Comments |
This item was isolated in cell culture by four passages in MDCK cells. |
| Effect on Host |
CPE, cell rounding and sloughing |
| Recommended Host |
MDCK (ATCC® CCL-34™) |
| Growth Conditions |
Temperature: 35°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 15 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-3 days |
| Name of Depositor |
ATCC |
| Source |
Specimen from a clinical patient in Virginia, 2009 |
