Restriction digests of the clone give the following sizes (kb): BamHI--7.4; HindIII--5.6, 1.8; SmaI--7.4. The vector contains the constitutive CaMV promoter adjacent to the tet operator. The vectors pSLF101 and pSLF102 differ in that pSLF101 has LEU2 marker and pSLF102, ura4+. A tet repressor must be supplied to regulate expression. Schizosaccharomyces pombe FY191 (ATCC 201437) has a tet repressor gene and integrating vector pSLF104 (ATCC 87621) contains the sup3-5 marker and tet repressor under adh promoter. |
