| 產(chǎn)品名稱 |
pEthr1 |
| 商品貨號 |
B185541 |
| Designations |
pEthr1 |
| Depositors |
Kyowa Ferm. Ind. Co., Ltd., S Wakaki, Kyowa Ferm. Ind. Co., Ltd. |
| U.S. Patent |
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level |
1 |
| Host |
Distribution host: Corynebacterium glutamicum LA201 |
| Vector Information |
Size (kb): 15.6000003814697300 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCG11
Intact vector size: 6.700
Type of vector: plasmid
Vector end: BglII
Vector end: BglII
Cloning sites: PstI BglII
Polylinker sites:
Construction: pCG1, pCG4
Host range: Brevibacterium sp.; Corynebacterium sp.
Features (with orientation and position when available):
marker(s): spcR, strR
replicon: pCG4
Cross references: Vector: pEthr1 (plasmid) Construction: pCG11, pGH2 Marker(s):kanR,spcR Construct size (kb): 15.60000038146973 Features: marker(s): kanR, spcR
replicon: pCG4 |
| Applications |
contains sequence aspartokinase I-homoserine dehydrogenase I shuttle vector |
| Comments |
The construct contains the following restriction sites (in approximate kb from the unique PstI site): EcoRI--1.1, 4.1, 13.1; XhoI--7.3; HindIII--6.7. The original vials from the depositor are being used for distribution. pGH2 contains the threonine operon from Escherichia coli. The BamHI fragment of pGH2 in this construct encodes homoserine dehydrogenase. Transformed strains do not require homoserine. This was constructed by inserting a 8.8 kb BamHI fragment from pGH2 into the BglII site of pCG11 (ATCC 39022). |
| Media |
ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
|
| Growth Conditions |
Temperature: 30.0°C |
| References |
Katsumata R, et al. Novel vector plasmids. US Patent 4,710,471 dated Dec 1 1987
|
| Shipped |
frozen |
| Shipping Information |
Distributed: freeze-dried |
