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Construct size (kb): 6.5

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCR3
Intact vector size: 5.100
Type of vector: phagemid
Vector end: TA site
Vector end: TA site
Cloning sites: TA cloning between EcoRI sites
Polylinker sites: HindIII KpnI SacI BamHI SpeI XmaIII BstXI EcoRI EcoRI PstI
EcoRV BstXI NotI XhoI XbaI ApaI
Construction: pcDNA3
Features (with orientation and position when available):
promoter: T7, ->
MCS: HindIII/.../TA cloning site/.../ApaI, ->
promoter: SP6, <-
other: bovine growth hormone polyA signal, ->
replicon: pMB1
marker(s): kanR, <-
promoter: SV40, <-
replicon: SV40, <-
marker(s): ampR, <-
replicon: f1, ->
promoter: cytomegalovirus, ->
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: NKAT1*
Gene name: natural killer associated transcript 1
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.42
Cross references: DNA Seq. Acc.: L41267
Nucleotides 1-1420 of the insert correspond to
nucleotides 22-1441 of L41267.

Insert lengths(kb): 1.419999957084656

Gene product: natural killer associated transcript 1 [NKAT1*]

Restriction digests of the clone give the following sizes (kb): ClaI--6.8; EcoRI--5.4, 1.4; NotI--6.8; NotI/SacI--5.2, 0.96, 0.54, 0.084, 0.04; SacI--5.2, 1.1, 0.5, 0.084.

The insert contains the following restriciton sites (approximate kb from the 5' end): PstI--0.08, 0.48, 0.55, 0.84; SacI--0.49; ClaI--1.20.