Albert MJ, et al. Live attenuated human rotavirus vaccine. US Pat. 4,624,850 dated Nov 25, 1986.
Other MA-104 cells should also work.
child with gastroenteritis
VR-2105 is contaminated with bacteria; VR-1546 is a cleaned version of this same strain.
The virus must be grown in serum-free media containing trypsin and should be pre-treated with trypsin to activate the virus before inoculation. Cell monolayers must be washed before inoculation to remove any FBS that would inactivate the trypsin.
VR-1546 created by freeze-thawing VR-2105 three times, then sterile-filtering it through 0.45 micron filter one time and 0.2 micron filter two times before inoculating.
Sato K, et al. Isolation of human rotavirus in cell cultures: brief report.. Arch. Virol. 69: 155-160, 1981. PubMed: 6171239
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