| 產(chǎn)品名稱 |
p-hPrl |
| 商品貨號(hào) |
B185656 |
| Designations |
p-hPrl |
| Species |
Homo sapiens, human |
| Depositors |
University of California, KH Murashige, University of California |
| Applications |
For growth of the host strain, the medium must contain DAP (diaminopimelic acid) (100 mg/L) and thymidine (20 mg/L). To detect the BglII polymorphism, a 203 bp EcoRI fragment containing exons 1 and 2 was used. |
| Vector |
Construct size (kb): 5.360000133514404 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: PstI
Vector end: PstI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: PstI
Vector end: PstI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749 |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: PRL
Genomic copy number: unique
Gene name: prolactin
Contains complete coding sequence?: Y
Chromosome: 6; Localization: 6 p22.2-p21.3
Tissue: pituitary adenoma
Type of DNA: cDNA
Insert end: Modification: GC tails
Insert end: Modification: GC tails
Insert size (kb): 0.959
Cross references: DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: PRL
Genomic copy number: unique
Gene name: prolactin
Contains complete coding sequence?: Y
Chromosome: 6; Localization: 6 p22.2-p21.3
Tissue: pituitary adenoma
Type of DNA: cDNA
Insert end: Modification: GC tails
Insert end: Modification: GC tails
Insert size (kb): 0.959
Cross references: Insert lengths(kb): 0.9589999914169312 Tissue: pituitary adenoma Gene product: prolactin [PRL] Alleles: A1, A2 |
| Insert Size (kb) |
0.959 |
| Media |
ATCC® Medium 1083: LB medium for chi 1776
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): PstI--4.4, 0.6, 0.3. For growth of the host strain, the medium must contain DAP (diaminopimelic acid) (100 mg/L) and thymidine (20 mg/L). Includes a synthetic poly(G) tract of 27 nt at the 5' end, and a poly(C) tract of 18 bp at the 3' end. The poly(A) is 81 nt. Contains the complete coding sequences with no codon changes from wild type. To detect the BglII polymorphism, a 203 bp EcoRI fragment containing exons 1 and 2 was used. |
| References |
Cooke NE, et al. Human and rat prolactin and preprolactin cloned genes. US Patent 4,725,549 dated Feb 16 1988
Myal Y, et al. A BglII RFLP at the human prolactin gene locus on chromosome 6 (PRL). Nucleic Acids Res. 19: 1167, 1991. PubMed: 1673564
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
