Restriction digests of the clone give the following sizes (kb): HindIII--4.5, 2.7; BamHI--2.7 (doublet), 1.8; PstI--2.9, 2.7, 1.0, 0.6; EcoRI--4.5, 2.7. Plasmid containing an oriT/kanR cassette bounded by multiple restriction sites for ease in subcloning and for selection in a broad range of gram-negative bacteria. The order of elements in the polylinker is: EcoRI SmaI BamHI XbaI PstI - HindIII insert oriT BamHI SalI PstI kanR/PstI insert HindIII - PstI XbaI BamHI SmaI KpnI EcoRI. |
