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pGC26
pGC26
規格:
貨期:
編號:B186080
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pGC26
商品貨號 B186080
Designations pGC26
Depositors JD Boeke
Biosafety Level 1
Vector Information
Size (kb): 4.6100001335144040
Vector: pGC26 (phagemid)
Promoters: Promoter for in vitro transcription T3
Construction: pRS402 pJK142 pRS421
Marker(s):ampR
Construct size (kb): 4.610000133514404
Features: marker(s): ampR
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: SacI/.../KpnI
coding sequence: lacZ'
Applications
YE-type (episomal) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.8; EcoRI--4.8; XbaI--3.3, 1.5.
This vector lacks a selectable marker in yeast. It will contribute many useful features as a base vector when used to construct new vectors that possess yeast selectable markers.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801

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梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479