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0.1% Gelatin Solution
0.1% Gelatin Solution
規(guī)格:
貨期:
編號:B186408
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 0.1% Gelatin Solution
商品貨號 B186408
Biosafety Level N/A
Applications
0.1% Gelatin Solution is used to coat cell culture flasks.
Product Format 100 ml
Storage Conditions 2 to 8°C
Comments
The use of coated flasks improves cell attachment for certain types of primary cells as well as certain continuous cell lines. This sterile-filtered solution contain 0.1% porcine gelatin in water.
Complete Growth Medium Preparing Gelatin-Coated Tissue Culture Flasks

For optimal cell attachment, cell culture flasks should be prepared before thawing the cells.

  1. Decontaminate the external surface of the 0.1% Gelatin Solution bottle by spraying with 70% ethanol.
  2. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, add 1.0 mL of 0.1% Gelatin Solution per 10 cm2 of culture surface area (e.g., 2.5 mL if using a T-25 flask). 
  3. Rock culture flask to coat surface; place in a 37°C incubator (with or without 5% CO2) for at least 30 minutes, and up to overnight.
  4. Aspirate the excess gelatin solution from the culture flasks using sterile technique.
  5. Add 5.0 mL of complete growth medium per 25 cm2 of culture surface area (e.g., 5.0 mL if using a T-25 flask).
  6. Place the gelatin-coated flasks in a 37°C, 5% CO2 incubator for at least one hour to equilibrate before inoculating with the cell suspension.   
Volume 100 ml
Sterility Tests
Negative for bacteria, fungi and yeast
Functional Tests Each lot is assessed for acceptable attachment of human primary cells.
A Certificate of Analysis (COA) is available upon request for each lot of 0.1% Gelatin Solution.
C of A
Certificate of Analysis
Certificate of Analysis
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479