| 產品名稱 |
pMON3215 |
| 商品貨號 |
B186703 |
| Designations |
pMON3215 |
| Species |
Bos taurus, cow |
| Depositors |
Monsanto Co., CH Clayman, Monsanto Co. |
| Applications |
produces protein growth hormone |
| Vector |
Construct Size (kb): 5.6
Name of vector: pBGHex-1
Intact vector Size:
Type of Vector: plasmid
Vector End: XbaI
Vector End: XbaI
Cloning Sites:
Polylinker Sites:
Host Range: Escherichia coli
Features (with orientation and position when available):
Promoter for Expression:
Coding Sequence: bGH
Marker(s): tetR
Replicon: pMB1
Marker(s): ampR
|
| Insert |
Genome: bovine
Gene Symbol: GH
Gene Name: growth hormone
Contains Complete Coding Sequence?: Y
Chromosome: 19; Localization: 19
Type of DNA: cDNA
Insert End: Modification: XbaI linker
Insert End: Modification: XbaI linker
Insert Size (kb): 0.59 |
| Media |
ATCC® Medium 6025: LB Medium with 12 mcg/ml tetracycline
ATCC® Medium 6026: LB Medium with 200 mcg/ml Ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--5.6; SmaI--5.6; XbaI--5.0, 0.6. Expresses a variant without an N-terminal Met--namely with an N-terminal Ala (aa -1)--and with Val at aa 126. |
| References |
Krivi GG. Production of proteins in procaryotes. US Patent 5,399,489 dated Mar 21 1995
Krivi GG. Biologically active method of using somatotropins. US Patent 5,037,806 dated Aug 6 1991
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
