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p2-47-4
p2-47-4
規(guī)格:
貨期:
編號:B187202
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 p2-47-4
商品貨號 B187202
Designations p2-47-4
Species Turnip crinkle virus
Depositors AE Simon
Vector
Construct size (kb): 3.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 0.699999988079071
Gene product: satellite RNA C
Insert Size (kb) 0.700
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
The insert contains internal SmaI, NcoI, DraI, SpcI and BstEII sites.
Restriction digests of the clone give the following sizes (kb): BamHI--3.4; HindIII--3.4; EcoRI--3.4; HindIII/EcoRI--3.2, 0.70.
The insert can be transcribed in vitro to yield infectious satellite cRNA.
Contains a head to tail dimer of RNA C cDNA.
Classification Carmovirus
References

Simon AE, Howell SH. Synthesis in vitro of infectious RNA copies of the virulent satellite of turnip crinkle virus. Virology 156: 146-152, 1987. PubMed: 3811230

Simon AE, Howell SH. The virulent satellite RNA of turnip crinkle virus has a major domain homologous to the 3' end of the helper virus genome. EMBO J. 5: 3423-3428, 1986.

Anne E Simon, personal communication

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