| 產品名稱 | Dicer1-/- |
|---|---|
| 商品貨號 | B187298 |
| Organism | Mus musculus, mouse |
| Tissue | Derived from: mesenchyme |
| Cell Type | mesenchymal stem cell |
| Product Format | frozen 1.0 mL |
| Morphology | mesenchymal-like |
| Culture Properties | adherent |
| Biosafety Level | 2 [Cells contain SV40 viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | 13 month |
| Gender | female |
| Applications | Dicer1-/- mesenchymal stem cells (ATCC® CRL-3221™) lack expression of the Dicer1 protein. Dicer1-/- cells can be used in conjunction with the matching Dicer1f/f cells (ATCC® CRL-3220™) to study the role of Dicer and miRNA in cell biology. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | The cells were first derived as primary cultures from a Dicer1f/f mouse with loxP sites present on either side of exon 23 of the Dicer1 gene. The primary culture was then infected with ecotropic virus encoding SV40 large T-Antigen, and immortalized. The immortalized MSCs then were infected with Adeno-Cre-GFP to introduce Dicer gene knockout by Cre/loxP- mediated recombination. The cells were sorted for GFP. Clones were then isolated and genotyped. |
| Genes Expressed | functional Dicer1 not expressed |
| Comments | MicroRNAs (miRNAs) are a new class of small noncoding RNAs that post-transcriptionally regulate the expression of target mRNA transcripts. Dicer is an RNase III family endoribonuclease that has the biofunction of processing miRNAs. Loss of Dicer1 leads to a dramatic decrease in levels of cellular microRNA, allowing for investigations into the roles of microRNAs in various cellular functions. CRL-3221 the Dicer1-/- cell line is a model of homozygous Dicer1-deletion in murine mesenchymal stem cells established from an adult Dicer1f/f mouse and immortalized in vitro. CRL-3220 is the Dicer1f/f immortalized mouse mesenchymal stem cell line that can be paired with CRL-3221 and used as a control. |
| Complete Growth Medium | Alpha minimum essential medium with ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Medium Renewal: 2 to 3 times per week Seeding Density: 1.5 x 104 to 3.0 x 104 cells/cm2 |
| Cryopreservation | Freeze Medium: Fetal Bovine Serum (FBS), 92%; DMSO, 8% Storage Temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature:37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Volume | 1.0 mL |
| Name of Depositor | A Gurtan (P Sharp's lab) |
| Year of Origin | 2009 |
| References | Ravi A, et al. Proliferation and tumorigenesis of a murine sarcoma cell line in the absence of DICER1. Cancer Cell 21(6): 848-855, 2012. PubMed: 22698408 Gurtan AM, et al. In vivo structure-function analysis of human Dicer reveals directional processing of precursor miRNAs. RNA 18: 1116-1122, 2012. PubMed: 22546613 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
