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yRM2123 [HTY3153]
yRM2123 [HTY3153]
規(guī)格:
貨期:
編號(hào):B187308
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 yRM2123 [HTY3153]
商品貨號(hào) B187308
Designations yRM2123 [HTY3153]
GenBank Number

U06152

U06154

U06155

Species Homo sapiens, human
Depositors HC Riethman
Applications
contains sequence DNA Segment, repetitive (telomere)
contains sequence DNA Segment, single copy
Vector
Construct size (kb): 270.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pTYAC1
Intact vector size: 9.000
Type of vector: YAC
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
restriction site: EcoRI
other: telomere, Tetrahymena, <-
marker(s): URA3
centromere: CEN4
replicon: ARS1
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D1Z9
Gene name: DNA Segment, repetitive (telomere)
Contains complete coding sequence?: Y
Chromosome: 1; Localization: 1 q44-qter
Genome: Homo sapiens
Gene symbol: D1S555
Gene name: DNA Segment, single copy
Contains complete coding sequence?: Y
Chromosome: 1; Localization: 1 q44
Tissue: lymphoblastoid CGM-1 cell line
Type of DNA: genomic
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 260.0
Cross references: DNA Seq. Acc.: U06152
DNA Seq. Acc.: U06154
DNA Seq. Acc.: U06155
Insert lengths(kb): 260.0
Tissue: lymphoblastoid CGM-1 cell line
Gene product: DNA Segment, repetitive (telomere) [D1Z9]
Target Gene: DNA Segment, single copy, DNA Segment, repetitive (telomere)
Insert Size (kb) 260.0
Media AHC- (AHC plus 40 mg/L tryptophan)
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen
Comments
yRM2123 shows some mitotic instability, so clones with the 270-kb YAC should be selected for additional manipulation.
Contains at least one CpG island. The sequence of this region is in accession U06154.
References

Negorev DG, et al. Physical analysis of the terminal 270 kb of DNA from human chromosome 1q. Genomics 22: 569-578, 1994. PubMed: 8001968

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