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Size (kb): 13.6300001144409200

Vector: pPbac (phagemid)

Promoters: Promoter polyhedrin

Construction: pJVP10Z

Marker(s):ampR

Construct size (kb): 13.63000011444092

Features: marker(s): ampR
other: 5'-SmaI-Stuffer fragment-BamHI-3'
promoter: P10
promoter: polyhedrin
replicon: f1
signal peptide coding region: human placental alkaline phosphatase
coding sequence: lacZ

expression vector

vector permitting visual detection of recombinants

vector with sequences for protein export

Restriction digests of the clone give the following sizes (kb): BamHI--14.0; EcoRI--14.0; HindIII--8.0, 4.3, 1.0, 0.9.

The gene of interest can be inserted into pPbac by removal of the stuffer fragment which has a 5' SmaI site and a 3' BamHI site.

pPbac is a baculovirus transfer vector that facilitates secretion of recombinant proteins in insect cell systems based on the presence of the human placental alkaline phosphatase leader signal.

Temperature: 37.0°C