| 產品名稱 |
pFV17 |
| 商品貨號 |
B187658 |
| Designations |
pFV17 |
| Species |
Saccharomyces cerevisiae, 2 micron circle |
| Depositors |
JR Broach |
| Applications |
in another host, produces protein recombinase, site-specific |
| Vector |
Construct size (kb): 6.800000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749 |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiae, 2 micron circle
Gene symbol: FLP
Genomic copy number: unique
Gene name: recombinase, site-specific
Contains complete coding sequence?: U
Type of DNA: genomic
Insert end:
Insert end:
Insert size (kb):
Cross references: Gene product: recombinase, site-specific [FLP] Target Gene: recombinase, site-specific |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--6.8; EcoRI--3.4, 2.15, 1.25; SalI--6.8; SalI/HindIII--5.9, 0.9; SalI/XbaI--5.3, 1.5. Linearize with BstEII before transformation into S. cerevisiae hosts. Integrating plasmid for galactose-inducible expression of FLP (from the GAL10 promoter). Constructed by cloning the HaeIII/HindIII fragment from the 2 micron circle into the SalI/HindIII sites of YEp51 (using a SalI linker), modifying the region between GAL10 and FLP to increase expression, and deleting the 2 micron origin of replication. Derived from pBR322 via YEp51. |
| References |
Rose AB, Broach JR. Propagation and expression of cloned genes in yeast: 2-micron circle-based vectors. Methods Enzymol. 185: 234-279, 1990. PubMed: 2199781
Volkert FC, Broach JR. Site-specific recombination promotes plasmid amplification in yeast. Cell 46: 541-550, 1986. PubMed: 3524855
|
