| 產品名稱 |
NKAT1 |
| 商品貨號 |
B187739 |
| Designations |
NKAT1 |
| GenBank Number |
L41267
|
| Species |
Homo sapiens, human |
| Depositors |
M Colonna |
| Vector |
Construct size (kb): 6.5 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCR3
Intact vector size: 5.100
Type of vector: phagemid
Vector end: TA site
Vector end: TA site
Cloning sites: TA cloning between EcoRI sites
Polylinker sites: HindIII KpnI SacI BamHI SpeI XmaIII BstXI EcoRI EcoRI PstI
EcoRV BstXI NotI XhoI XbaI ApaI
Construction: pcDNA3
Features (with orientation and position when available):
promoter: T7, ->
MCS: HindIII/.../TA cloning site/.../ApaI, ->
promoter: SP6, <-
other: bovine growth hormone polyA signal, ->
replicon: pMB1
marker(s): kanR, <-
promoter: SV40, <-
replicon: SV40, <-
marker(s): ampR, <-
replicon: f1, ->
promoter: cytomegalovirus, ->
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: NKAT1*
Gene name: natural killer associated transcript 1
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.42
Cross references: DNA Seq. Acc.: L41267
Nucleotides 1-1420 of the insert correspond to
nucleotides 22-1441 of L41267. Insert lengths(kb): 1.419999957084656 Gene product: natural killer associated transcript 1 [NKAT1*] |
| Insert Size (kb) |
1.420 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): ClaI--6.8; EcoRI--5.4, 1.4; NotI--6.8; NotI/SacI--5.2, 0.96, 0.54, 0.084, 0.04; SacI--5.2, 1.1, 0.5, 0.084. The insert contains the following restriciton sites (approximate kb from the 5' end): PstI--0.08, 0.48, 0.55, 0.84; SacI--0.49; ClaI--1.20. |
| References |
Colonna M, Samaridis J. Cloning of immunoglobulin-superfamily members associated with HLA-C and HLA-B recognition by human natural killer cells. Science 268: 405-408, 1995. PubMed: 7716543
|
