This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level
1
Host
Distribution host: Streptomyces lividans
Vector Information
Size (kb): 11.30
Vector: pGH202 (plasmid)
Construction: pEHB1.6 pIJ702
Marker(s):ampR tsrR
Construct size (kb): 11.30
Features: marker(s): ampR marker(s): tsrR replicon: pIJ101 replicon: pMB1 signal peptide coding region: endo H coding sequence: endo H
Applications
produces protein endo-beta-N-acetylglucosaminidase
shuttle vector
vector with sequences for protein export endo-beta-N-acetylglucosaminidase
Comments
Shuttle expression vector permitting secretion of fusion proteins from S. lividans hosts.
pGH202 (ATCC 39896) and pGH205 (ATCC 39895) differ in that the latter includes a promoter element for aminoglycoside phosphotransferase (aph).
S. lividans transformed with this plasmid will secrete endo H when grown in this medium: Difco yeast extract (3 g/L), Difco peptone (5 g/L), Oxoid malt extract (3 g/L), glucose (10 g/L), sucrose (340 g/L), and 5 mM MgCl2.
Media
YEME-thiostrepton medium
Growth Conditions
Temperature: 30.0°C
References
Guterman S. Streptomyces secretion vector. US Patent 4,745,056 dated May 17 1988
Robbins PW, et al. Primary structure of the Streptomyces enzyme endo-beta-N-acetylglucosamine. J. Biol. Chem. 259: 7577-7583, 1984. PubMed: 6429133
