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pTL-5495
pTL-5495
規(guī)格:
貨期:
編號(hào):B188495
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pTL-5495
商品貨號(hào) B188495
Designations pTL-5495
Species Tobacco etch virus
Depositors WG Dougherty
Vector
Construct size (kb): 7.159999847412109
Insert
DNA: cDNA
Insert lengths(kb): 4.079999923706055
Gene product: VPg, 6 kDa
Insert Size (kb) 4.080
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
SalI does not excise the insert. There is an internal SalI site in the insert.
Restriction digests of the clone give the following sizes (kb): BamHI--7.2; HindIII--3.3 (doublet) + other; SalI--4.9, 2.3; KpnI--7.2.
The vector (pTL-8) allows in vitro expression of the insert as a fusion protein.
The insert includes TEV cDNA sequences from nt 5413 to the 3' terminus of the genome at nt 9495.
Classification Potyvirus
References

Allison R, et al. The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein. Virology 154: 9-20, 1986.

Carrington JC, et al. Vectors for cell-free expression and mutagenesis of protein-coding sequences. Nucleic Acids Res. 15: 10066, 1987. PubMed: 3697076

Carrington JC, Dougherty WG. Small nuclear inclusion protein encoded by a plant potyvirus genome is a protease. J. Virol. 61: 2540-2548, 1987.

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