Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Comments
Note that activities with high potential for aerosol production require BSL 3 facilities and practices.
Add glassbeads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour. Feed with fresh growth medium containing 1-2 µg/mL cycloheximide. Incubate at 37°C for 48 hours.
Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa.
Recommended Host
McCoy (ATCC® CRL-1696™)
Growth Conditions
Temperature: 36°C Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 90-100% confluent. Prior to infection, disrupt cells by sonication or vortex with sterile glass beads. Remove medium and inoculate with a small volume of inoculum (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 1). Adsorb by centrifugation at 1500 x g for 1 hour at 25°C. End adsorption by adding growth medium. Incubation: 1-3 days
Name of Depositor
SP Wang
Source
Male urethra with non-gonococcal urethritis
References
Wang SP, et al. Three new immunologic types of trachoma-inclusion conjunctivitis organisms. J. Immunol. 110: 873-879, 1973. PubMed: 4631875
