| Insert |
DNA: cDNA Insert lengths(kb): 2.400000095367432 Tissue: liver Gene product: solute carrier family 2 (facilitated glucose transporter), member 2(glucose transporter 2) [GLUT2] Alleles: N1, N2, A1, A1, A2, A2, B1, B1, B2, B2, N1, N2, O2, A1, A1, A2, A2, B1, B1, B2, B2, O2, O2, O2 |
| Comments |
The insert includes the following restriction sites (nucleotide position according to the published sequence): SacI--451; HindIII--812; XmnI--1208; PstI--1525; EcoRI--1530. Restriction digests of the clone give the following sizes (kb): SalI--2.9, 2.4; EcoRI--3.8, 1.5; HindIII--3.6, 1.7; BamHI--5.3; PstI--4.4, 0.9; consistent with deposited DNA. The insert contains the complete coding sequence, including nucleotides 31-2471 of the published sequence. This probe detects transcripts of 2.8, 3.4, and 5.4 kb, which appear to differ in the lengths of the 3' untranslated regions. HTL-1, which contains a subset of the sequences in this clone, hybridized to human BamHI fragments of 12.0 and 6.7 kb, a mouse BamHI fragment of 15.0 kb, and a human EcoRI fragment of 8.3 kb. The 5' end of the insert is adjacent to the SP6 promoter. |
| References |
Fukumoto H, et al. Sequence, tissue distribution, and chromosomal localization of mRNA encoding a human glucose transporter-like protein. Proc. Natl. Acad. Sci. USA 85: 5434-5438, 1988. PubMed: 3399500
Chappell PB, et al. Polymorphism at the D20S13 (pphi64) locus. Nucleic Acids Res. 18: 4956, 1990. PubMed: 1975666
Horsthemke B, et al. Two EcoRI RFLPs at the GLUT2 locus. Nucleic Acids Res. 18: 6175, 1990. PubMed: 1978290
Froguel P, et al. Two TaqI RFLPs at the GLUT2 locus in French caucasian population. Nucleic Acids Res. 19: 5799, 1991. PubMed: 1682886
Graeme I Bell, personal communication
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