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pBSL141
pBSL141
規格:
貨期:
編號:B189086
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pBSL141
商品貨號 B189086
Designations pBSL141
Depositors MF Alexeyev
Biosafety Level 1
Vector Information
Size (kb): 3.8859999179840090
Vector: pBSL141 (phagemid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construction: pBluescript
Marker(s):ampR,gtmR
Construct size (kb): 3.885999917984009
Features: marker(s): ampR
promoter for in vitro transcription: T7 (phi10)
replicon: f1
replicon: pBM1
MCS: KpnI...SacI MluI
MCS: MluI KpnI...SacI
coding sequence: aacC1 (gtmR)
Applications
contains easily purifiable cassette(s) for construction gentamicin acetyltransferase
Comments
Restriction digests of the clone give the following sizes (kb): MluI--3.1, 0.8; BamHI--2.9, 1.0; NotI--2.9, 1.0.
Problems have been reported when using DH5alpha as a host for multicopy plasmids bearing Gm and Tc, but not when TG1 is used.
Plasmid useful for vector construction. Contains a gentamicin acetyltransferase (aacC1, gtmR) cassette flanked by multiple cloning sites duplicated in tandem orientation.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718

Mikhail F Alexeyev, personal communication

Shipped frozen
Shipping Information

?Frozen glycerol stock of E. coli containing the phagemid
梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479