| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--5.4, 2.7; PstI--4.9, 2.6, 1.7 (doublet); BamHI--12.0; HindIII--5.2, 2.7, 2.1, 0.8; BglI--10.0, 1.8; EcoRI/HindIII--3.4, 2.4 (doublet), 0.85. The plasmid contains two copies of the insert. |