Wild type strains: yeast strains used to make gene pool libraries (AA3)
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Type Strain
no
Genotype
MATalpha clp1-L SUC2 mal mel gal2 CUP1 trp1(ochre mutation) [rho0]
Preceptrol®
no
Mating Type
alpha
Ploidy
haploid
Comments
Wild type strains: yeast strains used to make gene pool libraries (AA3)
Used to make the lambda yeast library
Medium
ATCC® Medium 1069: YPAD medium
Growth Conditions
Temperature: 25.0°C
Atmosphere: Typical aerobic
Name of Depositor
YGSC
Special Collection
Yeast Genetic Stock Center
Chain of Custody
ATCC <<--YGSC<<--M.V. Olson
Isolation
Ethidium bromide-induced [rho0] mutant derived from X2180-1B (ATCC 204655 with trp1(ochre mutation) supplied by E.W. Jones). The lineage of AB972 traces directly to S288C (ATCC 204508) with no intervening outcrosses.
References
Olson MV, et al. Random-clone strategy for genomic restriction mapping in yeast. Proc. Natl. Acad. Sci. USA 83: 7826-7830, 1986. PubMed: 3463999
