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Size (kb): 7.6999998092651370

Vector: pBRH3B (plasmid)

Promoters: Promoter none

Construction: pBR316

Marker(s):ampR

Construct size (kb): 7.699999809265137

Features: insert detection: tetR
marker(s): ampR
promoter: none
replicon: pMB1

promoter-cloning vector

This is a promoter cloning plasmid vector using expression of tetracycline resistance for selection.

This was constructed by inserting an oligonucleotide containing an EcoRI site into the HindIII site of pBR316, inactivating the tet promoter. It is resistant to low levels of tetracycline without an insert and can be used to clone only strong promoters.

Temperature: 37.0°C